TitleAnalysis of behavior and trafficking of dendritic cells within the brain during toxoplasmic encephalitis.
Publication TypeJournal Article
Year of Publication2011
AuthorsJohn, B, Ricart, B, Wojno, EDTait, Harris, TH, Randall, LM, Christian, DA, Gregg, B, De Almeida, DManzoni, Weninger, W, Hammer, DA, Hunter, CA
JournalPLoS Pathog
Date Published2011 Sep
KeywordsAdoptive Transfer, Animals, Brain, CD11c Antigen, Cell Movement, Dendritic Cells, Encephalitis, Lymphocyte Function-Associated Antigen-1, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microglia, Pertussis Toxin, Receptors, Chemokine, T-Lymphocytes, Toxoplasma, Toxoplasmosis, Cerebral

<p>Under normal conditions the immune system has limited access to the brain; however, during toxoplasmic encephalitis (TE), large numbers of T cells and APCs accumulate within this site. A combination of real time imaging, transgenic reporter mice, and recombinant parasites allowed a comprehensive analysis of CD11c+ cells during TE. These studies reveal that the CNS CD11c+ cells consist of a mixture of microglia and dendritic cells (DCs) with distinct behavior associated with their ability to interact with parasites or effector T cells. The CNS DCs upregulated several chemokine receptors during TE, but none of these individual receptors tested was required for migration of DCs into the brain. However, this process was pertussis toxin sensitive and dependent on the integrin LFA-1, suggesting that the synergistic effect of signaling through multiple chemokine receptors, possibly leading to changes in the affinity of LFA-1, is involved in the recruitment/retention of DCs to the CNS and thus provides new insights into how the immune system accesses this unique site.</p>

Alternate JournalPLoS Pathog
PubMed ID21949652
PubMed Central IDPMC3174247
Grant ListT32 AI07532 / AI / NIAID NIH HHS / United States
R01 AI071302 / AI / NIAID NIH HHS / United States
R21 AI090234 / AI / NIAID NIH HHS / United States
T32 AI007532 / AI / NIAID NIH HHS / United States
R01 AI082292 / AI / NIAID NIH HHS / United States